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KMID : 0356719980140010021
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Journal of the Korean Society of Coloproctology
1998 Volume.14 No. 1 p.21 ~ p.26
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A Preliminary Study on the Expression and Regulation of C-X-C Chemokine Genes in a Human Colon Epithelial Cell Line
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Yang Suk-Kyun
Kim Ok-Hee
Lee Mi-Hwa
Park Sun-Mi
Jung Hwoon-Yong
Hong Weon-Seon
Min Young-Il
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Abstract
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Human colon epithelial cells secrete an array of proinflammatory cytokines that includes IL-8, MCP-1, GM-CSF, TNFα and IL-6. This response may serve to attract neutrophils and macrophags to the site of infection. In addition to IL-8 and MCP-1, the chemokine family contains other members, which, alone or in combination, can recruit and/or activate inflammatory and lymphoid cells. In this study, we asked whether colon epithelial cells express a broader array of chemokines than previously described. The colon epithelial cell line, Caco-2, was stimulated for 3h with IL-1α, or was infected with Salmonella dublin. RNA was extracted and chemokine mRNA levels were determined by quantitative reverse transcription-PCR using internal RNA standards. Ex pression of GROα, GROβ, GROβ and IP-10 increased by bacterial infection or IL-lα stimulation. These data strongly support the notion that epithelal cells are an important and integral component of the host’s natural immune system.
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KEYWORD
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Chemokine, Epithelial cell, Colon
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